Cleft palate cells can regenerate a palatal mucosa in vitro.
نویسندگان
چکیده
Cleft palate repair leaves full-thickness mucosal defects on the palate. Healing might be improved by implantation of a mucosal substitute. However, the genetic and phenotypic deviations of cleft palate cells may hamper tissue engineering. The aim of this study was to construct mucosal substitutes from cleft palate cells, and to compare these with substitutes from normal palatal cells, and with native palatal mucosa. Biopsies from the palatal mucosa of eight children with cleft palate and eight age-matched control individuals were taken. Three biopsies of both groups were processed for (immuno)histochemistry; 5 were used to culture mucosal substitutes. Histology showed that the substitutes from cleft-palate and non-cleft-palate cells were comparable, but the number of cell layers was less than in native palatal mucosa. All epithelial layers in native palatal mucosa and mucosal substitutes expressed the cytokeratins 5, 10, and 16, and the proliferation marker Ki67. Heparan sulphate and decorin were present in the basal membrane and the underlying connective tissue, respectively. We conclude that mucosal cells from children with cleft palate can regenerate an oral mucosa in vitro.
منابع مشابه
Cytokeratin expression in palatal and marginal mucosa of cleft palate patients.
OBJECTIVE The margin of a palatal cleft is a unique anatomical site since the palatal mucosa is continuous with the nasal or nasopharyngeal mucosa. The aim of this study was to compare the expression patterns of cytokeratins and basal membrane components of the mucosa in the area of the cleft. DESIGN Biopsies from the mucosa of the hard palate and from the cleft margin in the soft palate were...
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Background Cleft palate (CP) is a common congenital defect. It makes serious difficulties for cleft-affected children. The gold standard of care is autogenous bone grafting which may cause additional problems in donor site along with disappointing results. Tissue engineering is a promising solution for a widespread range of defects and disorders. It is reasonable to utilize this novel technolo...
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Cleft of the primary and secondary palate in human beings is a frequent congenital malformation occurring once in 800 live births.' In Michigan, this congenital defect is incident once in 740 live births.2 Although both genetic and environmental forces are involved in cleft formation, the genetic mechanism has not been described adequately or have specific environmental agents been identified. ...
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ورودعنوان ژورنال:
- Journal of dental research
دوره 87 8 شماره
صفحات -
تاریخ انتشار 2008